lemur_laying _eggs_crarc

Lemur leaf frogs laying eggs at the CRARC               (c) Brian Kubicki

A major part of this project has also been to facilitate the professional management of a genetically-viable ex-situ captive population of Costa Rican Agalychnis lemur for the future. As PCR primers for microsatellites were lacking for this species, supporting the necessary research to develop a genetic marker system that would enable current captive specimens to be genetically defined for the programme was one key aim of this project. Alex Petchey, a Master’s student from Salford University in Manchester  conducted detailed DNA fingerprint research that has been highly successful. The work was supported exclusively by Project Lemur Frog. The genetic research was initiated in 2012 and PCR work was completed by the end of that year.  Genotyping commenced in 2013 with primers described and individuals then characterised.  As a result, an official EAZA ex-situ studbook has been established by Tim Skelton at Bristol Zoo. The related research publication describing the characterisation of 9 polymorphic micro-satellite markers for the species has now been published and is found below:

Research Paper in Conservation Genetics (July, 2014)

Other related research:

Gray A. R (2011). Notes on Hybridization in Leaf frogs of the genus Agalychnis   (Anura, Hylidae, Phyllomedusinae), Cornell University and the National Science Institutes’ ArXiv:1102.4039v1, can be viewed as a high quality printable PDF